USR PUBLISHER

Six alkaloid compounds were isolated (the discovery of a natural drug, which is a combination of the six alkaloid compounds). They are: 1) 1,3,5-Triazine-2-carboxylic acid, 4-amino-6-methyl 2) 1,2,4-Triazino[5,6-E][1,2,4]-triazine-3,6-dione, hexahydro 3) 2-(4,5-Dihydro-3-methyl-5-oxo-1-phenyl-4-pyrazol)-5-nitrobenzoic acid 4) 4-metyl-3,4-dihydro-[1,2,3] trizolo [4,5-d] pyrimidine-5,7-dione 5) Propanoic acid-3-(perhydro-2,5-dioxo-imidazol [4,5-d] imidazole-1-yl) 6) 1,2,3-Triazolo[4,5-f] benzotriazole- 4,8(4H,8H)-dione were isolated from Cinnamomumverumcortexesby using ethanolic acetic acid, sulphuric acid and ammonia solvents, and they were characterized by using of preliminary qualitative tests, gas chromatography-mass spectrumGC-MASS. The medicinal efficacy was studied for these six alkaloidic compounds theoretically by using theAutodock program and the results showed presence of high efficacy of the compounds. Then the medicinal efficacy was measured biologically for the six compounds mixture in vitro depending on synergistic interaction principle by using various concentrations (5, 10, 15, 20 and 25 mg / ml) by using of agar diffusiontechnique. The results showed that six alkaloidic compounds mixture is biologically active against Alternariaalternatefungus, where the inhibition zone diameters were recorded equal to 27, 33, 41, 45 and 49 mm respectively towards the pathogenic fungus.Also, cytoxicity tests for that different concentration of six active alkaloidic compounds mixture proved there is no toxiceffect belonging them towards red blood cells, and no hemolysis effect was appeared inblood cells. Therefore, six alkaloidic compounds isolated from Cinnamomumverumcortexescan be used as active drugfor treatment of various diseases especially caused by pathogenic fungus represented by Alternaria alternate, without any side effects.